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2019

Wu, Q., Takano, H., Riddle, D.M., Trojanowski, J.Q., Coulter, D.A., Lee, V.M.: α-Synuclein (αSyn) Preformed Fibrils Induce Endogenous αSyn Aggregation, Compromise Synaptic Activity and Enhance Synapse Loss in Cultured Excitatory Hippocampal Neurons. Journal of Neuroscience 39(26): 5080-5094, 2019.

(Fluorescence-guided patch-clamp recording system, integrating patch-clamp instrument with laser scanning confocal microscopy)

 

Usami Y, Gunawardena AT, Francois NB, Otsuru S, Takano H, Hirose K, Matsuoka M, Suzuki A, Huang J, Qin L, Iwamoto M, Yang W, Toyosawa S, Enomoto-Iwamoto M.: Possible Contribution of Wnt-Responsive Chondroprogenitors to the Postnatal Murine Growth Plate. J Bone Miner Res. 2019 Jan 2. doi: 10.1002/jbmr.3658. [Epub ahead of print] 2019. (Two-photon microscopy and tissue clearing method (CUBIC))

 

Tang S, Terzic B, Wang IJ, Sarmiento N, Sizov K, Yue CY, Takano H, Marsh ED, Zhou Z, Coulter DA.: Altered NMDAR signaling underlies autistic-like features in mouse models of CDKL5 deficiency disorder. Nature Communications  10(1): 2655, 2019. (Voltage Sensitive Dye (VSD) Imaging)

 

Kahn,J.B., Port,R.G., Yue,C., Takano,H., Coulter,D.A.: Circuit-based interventions in the dentate gyrus rescue epilepsy-associated cognitive dysfunction. Brain 142(9): 2705-2721, Sep 2019. (EEG analysis by Matlab coding)

2018

Mundy, C., Yang, E., Takano, H., Billings, P.C., Pacifici, M.: Heparan sulfate antagonism alters bone morphogenetic protein signaling and receptor dynamics, suggesting a mechanism in hereditary multiple exostoses. Journal of Biological Chemistry 293(20): 7703-7716, 2018.(Fluorescence Recovery After Photo-bleaching (FRAP))

2017

Tang, S., Wang, I.J., Yue, C., Takano, H., Terzic, B., Pance, K., Lee, J.Y., Cui, Y., Coulter, D.A., Zhou, Z.: Loss of CDKL5 in glutamatergic neurons disrupts hippocampal microcircuitry and leads to memory impairment in mice. Journal of Neuroscience 37(31): 0539, 2017. (VSD imaging)

Pearson-Leary, J., Eacret, D., Chen, R., Takano, H., Nicholas, B., Bhatnagar, S. : Inflammation and vascular remodeling in the ventral hippocampus contributes to vulnerability to stress. Transl Psychiatry 7(6): e1160, 2017. (Image Analysis, Matlab Coding)

Dengler, C.G., Yue, C., Takano, H., Coulter, D.A. : Massively augmented hippocampal dentate granule cell activation accompanies epilepsy development Scientific Report  2017(7): 42090, 2017. (Calcium Imaging,  Matlab Coding, Fast Confocal Microscopy (Sweptfield Microscopy))

2014

Shilling, D., Muller, M., Takano, H.,  Mak, D.O., Abel, T., Coulter. D.A., and Foskett, J.K.: Suppression of InsP3 receptor-mediated Ca2+ signaling alleviates mutant presenilin-linked familial Alzheimer's disease pathogenesis. Journal of Neuroscience 34(20): 6910-6923, 2014. (Fast Confocal Microscopy, Calcium Imaging, UV-Photolysis/uncaging)

Kuzum, D.*, Takano, H.*, Shim, E., Reed, J., Juul, H., Richardson, A., de Vries, J., Bink, H., Dichter, M., Lucas, T., Coulter, D.A., Cubukcu, E., and Litt, B.: Transparent, flexible, low noise graphene electrodes for simultaneous electrophysiology and neuroimaging. Nature Communications Page: 5259, 2014 Notes: online.  (* first co-author) (Graphene Electrode, Calcium Imaging)

Jackson, J.G., O'Donnell, J.C., Takano, H., Coulter, D.A., and Robinson, M.B.: Neuronal activity and glutamate uptake decrease mitochondrial mobility in astrocytes and position mitochondria near glutamate transporters. Journal of Neuroscience 34(5): 1613-1624, 2014.

2013

Yu, E., Dengler, C., Frausto, S., Putt, M., Yue, C., Takano, H., and Coulter, D.A.: Protracted postnatal development of sparse, specific dentate granule cell activation in the mouse hippocampus. Journal of Neuroscience  33(7): 2947-2960, 2013. (Calcium Imaging, VSD imaging)

Weerakkody, T.N., Patel, T.P., Yue, C., Takano, H., Anderson, H.C., Meaney, D.F., Coulter, D.A., Wolfe, J.H.: Engraftment of nonintegrating neural stem cells differentially perturbs cortical activity in a dose-dependent manner. Molecular Therapy Online, 2013. (VSD imaging)

2012

Takano, H., McCartney, M., Ortinski, P., Putt, M., and Coulter, D.A.: Deterministic and stochastic neuronal contributions to distinct synchronous CA3 network bursts. Journal of Neuroscience  32(14): 4743-4754, 2012. (Fast Confocal Microscopy (400Hz), Calcium Imaging, Image Analysis, Matlab Coding, Model Based Cluster Analysis)

2011

Coulter, D. A.,  Yue, C.,  Ang, C. W.,  Weissinger, F.,  Goldberg, E.,  Hsu, F. C.,  Carlson, G. C.,  Takano, H.: Hippocampal microcircuit dynamics probed using optical imaging approaches. J Physiol 589(Pt 8): 1893-903, 2011.4.

Takano, H.,Coulter, D.A.: Imaging of hippocampal circuits in epilepsy. Jasper's Basic Mechanisms of Epilepsies, 3rd Edition. Noebeis, J., Rogawski, M., Delgado-Escueta, A., Avoli, M. (eds.). 2011

2010 - 2002

Ortinski, P. I.,  Dong, J.,  Mungenast, A.,  Yue, C.,  Takano, H.,  Watson, D. J.,  Haydon, P. G.,  Coulter, D. A.: Selective induction of astrocytic gliosis generates deficits in neuronal inhibition. Nat Neurosci 13(5): 584-91, 2010. (Laser Confocal Microscopy)

Lin, H.,  Vicini, S.,  Hsu, F. C.,  Doshi, S.,  Takano, H.,  Coulter, D. A.,  Lynch, D. R.: Axonal alpha7 nicotinic ACh receptors modulate presynaptic NMDA receptor expression and structural plasticity of glutamatergic presynaptic boutons. Proc Natl Acad Sci U S A 107(38): 16661-6, 2010. (Laser Confocal Microscopy)

Potokar, M.,  Kreft, M.,  Lee, S. Y.,  Takano, H.,  Haydon, P. G.,  Zorec, R.: Trafficking of astrocytic vesicles in hippocampal slices. Biochem Biophys Res Commun 390(4): 1192-6, 2009.

Jin, L. Q.,  Zhang, G.,  Jamison, C., Jr.,  Takano, H.,  Haydon, P. G.,  Selzer, M. E.: Axon regeneration in the absence of growth cones: acceleration by cyclic AMP. J Comp Neurol 515(3): 295-312, 2009.(Two-Photon Microscopy)

Fellin, T.,  Halassa, M. M.,  Terunuma, M.,  Succol, F.,  Takano, H.,  Frank, M.,  Moss, S. J.,  Haydon, P. G.: Endogenous nonneuronal modulators of synaptic transmission control cortical slow oscillations in vivo. Proc Natl Acad Sci U S A 106(35): 15037-42, 2009.

Halassa, M. M.,  Fellin, T.,  Takano, H.,  Dong, J. H.,  Haydon, P. G.: Synaptic islands defined by the territory of a single astrocyte. J Neurosci 27(24): 6473-7, 2007.(Laser Confocal Microscopy)

Mrass, P.,  Takano, H.,  Ng, L. G.,  Daxini, S.,  Lasaro, M. O.,  Iparraguirre, A.,  Cavanagh, L. L.,  von Andrian, U. H.,  Ertl, H. C.,  Haydon, P. G.,  Weninger, W.: Random migration precedes stable target cell interactions of tumor-infiltrating T cells. J Exp Med 203(12): 2749-61, 2006. (Two-Photon Microscopy, 3D Image Analysis)

Fellin, T.,  Sul, J. Y.,  D'Ascenzo, M.,  Takano, H.,  Pascual, O.,  Haydon, P. G.: Bidirectional astrocyte-neuron communication: the many roles of glutamate and ATP. Novartis Found Symp 276: 208-17; discussion 217-21, 233-7, 275-81, 2006.

Barrett, L. E.,  Van Bockstaele, E. J.,  Sul, J. Y.,  Takano, H.,  Haydon, P. G.,  Eberwine, J. H.: Elk-1 associates with the mitochondrial permeability transition pore complex in neurons. Proc Natl Acad Sci U S A 103(13): 5155-60, 2006.

Barrett, L. E.,  Sul, J. Y.,  Takano, H.,  Van Bockstaele, E. J.,  Haydon, P. G.,  Eberwine, J. H.: Region-directed phototransfection reveals the functional significance of a dendritically synthesized transcription factor. Nat Methods 3(6): 455-60, 2006. (Two-Photon Microscopy)

Pascual, O.,  Casper, K. B.,  Kubera, C.,  Zhang, J.,  Revilla-Sanchez, R.,  Sul, J. Y.,  Takano, H.,  Moss, S. J.,  McCarthy, K.,  Haydon, P. G.: Astrocytic purinergic signaling coordinates synaptic networks. Science 310(5745): 113-6, 2005. (Photon-Counting CCD Camera, Bioluminescence)

2002 - 1997

Takano, H, Sul, JY, Mazzanti Y, Doyle, RT, Haydon, PG and Porter,MD: Micropatterned substrates: approach to probing intercellular communication pathways. Analytical Chemistry 74(18): 4640-4646, 2002. (Microfabrication, Soft-lithography, Calcium Imaging)

Takano, H., Porter, M. D.: Monitoring chemical transformations at buried organic interfaces by electric force microscopy. J Am Chem Soc 123(34): 8412-3, 2001.

Takano, H.,Porter, M.D.,Haydon, P.G.: Imaging the Spatial Organization of Calcium Channels in Nerve Terminals Using Atomic Force Microscopy. Ion Channel Localization: Methods and Protocols. Lopatin,A., Nichols,C.G.   (eds.). Humana Press  Page: 391-404, 2001.

 

Hajime Takano, Sze-Shun Wong, and Marc D. Porter: Depth Sensitivities in the Compositional Mapping of Organic Thin Films Using Scanning Force Microscopy. Langmuir 16: 5231-5233, 2000.

Takano,H,Wong,S.S., Harnisch,J.,Porter,M.D.: Chemically sensitive scanning force microscopy. Electrochemical Society 99(28): 46-54, 2000.

Porter, M.D., Takano, H.: Electrochemically Modulated Liquid Chromatography. Encyclopedia of Separation Science. Wilson,I.D., Adlard,E.R.,Cooke,M.,Poole,C.F.  (eds.). Academic Press, Page: 636-646, 2000.

Deng, H.,  Van Berkel, G. J.,  Takano, H.,  Gazda, D.,  Porter, M. D.: Electrochemically modulated liquid chromatography coupled on-line with electrospray mass spectrometry. Analytical Chemistry 72(11): 2641-7, 2000.

Takano,H., Kenseth,J.R., Wong, S.S., O'Brien,J.C., Porter, M.D.: Chemical and Biological Analysis using Scanning Force Microscopy. Chemical Review 99: 2845-2890, 1999.

 

Sze-Shun Wong, Hajime Takano, M. D. Porter: Mapping Orientation Difference by Friction Force Microscopy. Analytical Chemistry  70: 5209-5212, 1998.

1997 - 1992

Ivanov, G. R.,  Petkova, J. I.,  Okabe, Y.,  Aoki, D.,  Takano, H.,  Kawate, H.,  Fujihira, M.: Scanning probe microscopy studies of aggregation in Langmuir-Blodgett films. Supramolecular Science 4(3-4): 549-557, 1997.

Yamanaka, K.,  Takano, H.,  Tomita, E.,  Fujihira, M.: Lateral force modulation atomic force microscopy of Langmuir-Blodgett film in water. Japanese Journal of Applied Physics Part 1-Regular Papers Short Notes & Review Papers 35(10): 5421-5425, 1996.

 

Takano, H.,  Fujihira, M.: AFM microlithography of a thin chromium film covered with a thin resist Langmuir-Blodgett (LB) film. Thin Solid Films 273(1-2): 312-316, 1996.

 

Takano, H.,  Fujihira, M.: Study of molecular scale friction on stearic acid crystals by friction force microscopy. Journal of Vacuum Science & Technology B 14(2): 1272-1275, 1996.

 

Fujihira, M.,  Aoki, D.,  Okabe, Y.,  Takano, H.,  Hokari, H.,  Frommer, J.,  Nagatani, Y.,  Sakai, F.: Effect of capillary force on friction force microscopy: A scanning hydrophilicity microscope. Chemistry Letters(7), 499-500, 1996.

 

Fujihira, M.,  Takano, H.: Study on frictional-properties of Langmuir-Blodgett-films by friction force microscopy. Journal of Japanese Society of Tribologists 40(6): 470-475, 1995.

 

Overney, R. M.,  Takano, H.,  Fujihira, M.,  Paulus, W.,  Ringsdorf, H.: Ansiotropy in friction and molecular stick-slip motion. Phys Rev Lett 72(22): 3546-3549, 1994.

 

Overney, R. M.,  Takano, H.,  Fujihira, M.,  Meyer, E.,  Guntherodt, H. J.: Wear,friction and sliding speed correlations on Langmuir-Blodgett-films observed by atomic-force microscopy. Thin Solid Films 240(1-2): 105-109, 1994.

 

 

Overney, R. M.,  Takano, H.,  Fujihira, M.: Elastic compliances measured by atomic-force microscopy. Europhysics Letters 26(6): 443-447, 1994.

 

Overney, R. M.,  Meyer, E.,  Frommer, J.,  Guntherodt, H. J.,  Fujihira, M.,  Takano, H.,  Gotoh, Y.: Force microscopy study of friction and elastic compliance of phase-separated organic thin-films. Langmuir 10(4): 1281-1286, 1994.

 

Fujihira, M.,  Takano, H.: Observation of the phase-separated Langmuir-Blodgett-films on vapor- deposited silver films with atomic-force microscopy and friction force microscopy. Thin Solid Films 243(1-2): 446-449, 1994.

 

Fujihira, M.,  Takano, H.: Atomic-force microscopy and friction force microscopy of Langmuir-Blodgett-films for microlithography. Journal of Vacuum Science & Technology B 12(3): 1860-1865, 1994.

 

Overney, R. M.,  Meyer, E.,  Frommer, J.,  Brodbeck, D.,  Luthi, R.,  Howald, L.,  Guntherodt, H. J.,  Fujihira, M.,  Takano, H.,  Gotoh, Y.: Friction measurements on phase-separated thin-films with a modified atomic force microscope. Nature 359(6391): 133-135, 1992.

 

Meyer, E.,  Overney, R.,  Luthi, R.,  Brodbeck, D.,  Howald, L.,  Frommer, J.,  Guntherodt, H. J.,  Wolter, O.,  Fujihira, M.,  Takano, H.,  Gotoh, Y.: Friction force microscopy of mixed Langmuir-Blodgett-films. Thin Solid Films 220(1-2): 132-137, 1992.

ABOUT US

Dr. Takano, originally a nano-scale imaging expert in application of scanning probe microscopy to chemical and biological analysis, shifted his research direction after he saw a time-series images of "calcium wave" captured by fluorescence microscopy (2001). Since then his research focus has been in neuroscience research technology development, especially imaging instrumentation and analysis algorithm development.

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In Vivo Imaging

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Neuron

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Optics

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Analysis

R21 is funded from next month! The resubmission was somehow reviewed and got very good score. This was redundant information, but it was good to know...

R21 starting!

July 27, 2018

I received an email from NINDS requesting JIT document for the application I submitted almost a year ago. I ignored the automatic email request from N...

JIT

May 25, 2018

The cause of the laser failure was overheating due to accumulated dusts on the air-filter of the power supply unit. I have to make sure that the filte...

2P laser is finally repaired.

April 3, 2018

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