I have not had a chance to see Pinnacle's seizure detection analysis package until yesterday when one of researchers at Penn contacted me. Considering convenience of video reviewing process, Pinnacle's package is nice, and can do many basic analysis such as power calculation at different bands, seizure detection via power and/or linelength, and output to spreadsheet for further analysis. I am currently using custom-written Matlab code for seizure detection and the "feature" t
So, the estimates for the laser repair is $10k, including service visit fee and parts. They think it is a power supply failure inside of the controller. Considering the original price of the laser (~150k) and the cost of annual service contract fee (~10k per year; which I did not subscribe to), it is not bad, but it is hard to come up with 10k in this financial climate.
In my undergraduate research project, I learned about Förster resonance energy transfer. I was assigned to synthesize pyrene-based organic compounds and conducted fluorescence imaging. Fluorescence bleach started from small domain, and the hypothesis was that the oxidation product act as energy acceptor. 20 years fast-forward, I am conducting fluorescence lifetime imaging using two-photon microscopy. It seems like chloride indicator dye is serving as energy donor with tDtomat
On the last day of work last year (a week ago), I put the sample on the stage, setup the electrode for recording, and started to image, then the MaiTai laser controller suddenly shut off and started to smell like circuit-board burning! The fan shut off, LCD status indicator became blank, but the electric power was somehow still on. I shut everything off, and went into the holiday break. I finally rebuilt the working 2P system few months ago, but now the heart of the 2P imag